The plane was loaded from World Vision's Global Pre-positioning Unit warehouses in Brindisi, Italy, and arrived in Amman at 2.50pm.
World Vision staff in Brindisi and Amman worked feverishly over the past two days to ensure the plane would arrive on time.
World Vision's partner Red Crescent, who will help distribute some of the supplies in the event of a refugee crisis, has set a deadline of March 16 for the relief goods to be in place.
World Vision Middle East vice-president Dave Robinson, who was on the flight, described it as a great success.
"It was an impressive, well co-ordinated and executed delivery involving multiple partners," he said.
"The World Vision partnership's investment in pre-positioning paid off today."
Mr Robinson noted the generosity of World Vision Hong Kong and Taiwan who donated the cost of the flight and goods.
He also commended the co-operation of WFP who helped with organising the loading of the plane at Brindisi, and World Vision's partners Caritas Jordan and the Red Crescent.
Relief supplies on the plane will be used to assist refugees fleeing Iraq in the event of war. Some will be retained for use in Iraq itself should conditions permit.