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Between epidemiological week (EW) 1 and EW 44 of 2024, 50 confirmed cases of yellow fever in humans have been reported in the Americas Region, including 24 deaths. This represents 12 additional cases (one in the Plurinational State of Bolivia, nine in Colombia, one in Guyana, and one in Peru) and five additional deaths (one in Bolivia and four in Colombia) since the previous Pan American Health Organization/World Health Organization (PAHO/WHO) yellow fever epidemiological update published on 19 September 2024.