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Given the increase in human cases of yellow fever in the last months of 2024 and beginning of 2025 in countries of the Americas Region, the Pan American Health Organization / World Health Organization (PAHO/WHO) calls on Member States with areas at risk to continue their efforts to strengthen surveillance in endemic areas, vaccinate populations at risk, and take the necessary actions to ensure that travelers going to areas where vaccination is recommended are properly informed and protected against yellow fever. In addition, it highlights the need to strengthen clinical management, with emphasis on the detection and timely treatment of severe cases. PAHO/WHO also recommends having reserve doses, in accordance with the availability of vaccines in each country, to guarantee a rapid response to possible outbreaks.